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GrainGenes Probe Report: WMC539

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC539  [ Marker Report ]
Locus	Xwmc539 Xwmc539-6B wmc539 wmc539-6B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26a6 Donor: Madre, Secobra Trimmed to insert at both ends. (GT)64 75 to 202.
Type	PCR SSR
PCR primers	Forward gcAAgTAggAccTTAcAgTTcT at 27 length 22 bases, Tm 56 Reverse gTTATAAccTTTgTcccTTcAc at 204 length 22 bases, Tm 56
SSR size	199 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product (200 bases).
Sequence	WMC539
Source Species	Triticum aestivum