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GrainGenes Probe Report: WMC538

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC538
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26h4 Donor: Madre, Secobra Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 323 to 374 (complement) show homology to 17f9 bases 50 to 101 (52 in longest run, maxScore = 79), primers have no apparent match to 17f9. (GT)17 15 to 48.
Type	PCR SSR
PCR primers	Forward AgccATTgAAATTgTgT at 2 length 17 bases, Tm 51 Reverse TgATcTATgcccTcTgA at 87 length 17 bases, Tm 52
SSR size	102 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product (80 bases)
Sequence	WMC538
Source Species	Triticum aestivum