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GrainGenes Probe Report: WMC536

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC536
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26d2 Donor: Madre, Secobra Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 1 to 302 show homology to 26e2 bases 1 to 302 (302 in longest run, maxScore = 190). (GT)32 193 to 256. Duplicate with 26e2, 26d2 has priority.
Type	PCR SSR
PCR primers	Forward AAcATTTAAcccTAcAATgccA at 55 length 22 bases, Tm 60 Reverse TAAcTAggATgcAcgcAgTcAA at 261 length 22 bases, Tm 62
SSR size	228 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product.
Sequence	WMC536
Source Species	Triticum aestivum