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GrainGenes Probe Report: WMC535

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC535
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26c2 Donor: Madre, Secobra Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 283 to 358 show homology to 5e3 bases 138 to 213 (76 in longest run, maxScore = 136). Reverse primer has 3 mismatches to 5e3, forward primer sequence is not in 5e3. (GA)11 115 to 136, (GT)6 347 to 358, (GT)5 371 to 380, (GT)5 385 to 394.
Type	PCR SSR
PCR primers	Forward ATgTTTgATgAgAgAgcTggAA at 7 length 22 bases, Tm 61 Reverse ATgTAAcAAcggcTgAcTgAcT at 153 length 22 bases, Tm 61
SSR size	168 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear (120-160 bases). 51C anneal, product.
Sequence	WMC535
Source Species	Triticum aestivum