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GrainGenes Probe Report: WMC528

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC528
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24C10 Donor: Bright, Zeneca Trimmed to insert at 5' end , 3' end not located. Bases 92 to 148 show homology to 10A1.2 bases 90 to 146 (57 in longest run, maxScore = 66). Reverse primer perfect match to 10a1.2, forward primer no match. (GT)5GC(GT)5 32 to 53, (GT)4 70 to 77.
Type	PCR SSR
PCR primers	Forward AAgATATTccTATTcTATgAAccT at 8 length 24 bases, Tm 54 Reverse TcATAcAcTAcAcAccATcTcT at 111 length 22 bases, Tm 54
SSR size	125 bases expected, Chinese Spring
Amplification Conditions	51C anneal, smear.
Sequence	WMC528
Source Species	Triticum aestivum