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GrainGenes Probe Report: WMC527

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC527  [ Marker Report ]
Locus	Xwmc527 [ Show all 10 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24B10 Donor: Bright, Zeneca Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 378 bases located on 3B Trimmed to insert at 5' end and to Ssp1 at 3' end. (CA)15 328 to 357, (GA)20 30 to 69.
Type	PCR SSR
PCR primers	Forward AcccAAgATTggTTgcAgAA at 4 length 20 bases, Tm 63 Reverse gcTAcAgAAAAccggAgccTAT at 366 length 22 bases, Tm 62
SSR size	386 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product (360 bases).
Sequence	WMC527
Source Species	Triticum aestivum