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GrainGenes Probe Report: WMC526

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC526  [ Marker Report ]
Locus	Xwmc526 Xwmc526-7B wmc526
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24A10 Donor: Bright, Zeneca Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 188 bases located on 7B Trimmed to insert at 5' end, 3' end not located. Bases 81 to 161 (complement) show homology to 25e7 bases 114 to 194 (81 in longest run, maxScore = 119). Reverse primer completely homologous to 25e7, forward primer is not in 25e7 sequence. Bases 235 to 352 show homology to 26a1 bases 82 to 199 (complement) (118 in longest run, maxScore = 193). (CA)33 79 to 144. Duplicate with 26a1, 24a10 has priority.
Type	PCR SSR
PCR primers	Forward TcccATTggTTcAcAAAcTcg at 58 length 21 bases, Tm 65 Reverse gATggTATcgcATTcATcggT at 225 length 21 bases, Tm 64
SSR size	188 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (150-190).
Sequence	WMC526
Source Species	Triticum aestivum