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GrainGenes Probe Report: WMC524

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC524  [ Marker Report ]
Locus	Xwmc524-5A Xwmc524 wmc524 wmc524-5A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 22f6 Donor: Bright, Zeneca Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 207 bases located on 5A Trimmed to insert at both ends. Bases 204 to 215 (complement) show homology to 3e11 bases 124 to 135 (12 in longest run, maxScore = 19). (GT)13 233 to 258, (GT)7 261 to 274, (GT)4 316 to 323.
Type	PCR SSR
PCR primers	Forward TAgTccAccggAcggAAAgTAT at 165 length 22 bases, Tm 63 Reverse gTAccAccgATTgATgcTTgAg at 341 length 22 bases, Tm 63
SSR size	198 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (160-200 bases).
Sequence	WMC524
Source Species	Triticum aestivum