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GrainGenes Probe Report: WMC521

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC521
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 22e5.2 Donor: Bright, Zeneca No overlap between forward and reverse sequences. Trimmed to Ssp1 at 3' end, 5' end not located. This is the reverse sequence. Bases 110 to 118 show homology to 22a3.2 bases 164 to 172 (9 in longest run, maxScore = 57). (CA)20 772 to 811.
Type	PCR SSR
PCR primers	Forward TccccgTcAATgggTTTTT at 718 length 19 bases, Tm 64 Reverse TATTTgAcATcTcAAcAATgAcTTT at 811 length 25 bases, Tm 58
SSR size	116 bases expected, Chinese Spring
Amplification Conditions	tested 51C anneal, no product.
Sequence	WMC521
Source Species	Triticum aestivum