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GrainGenes Probe Report: WMC520

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC520
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 22a5 Donor: Bright, Zeneca Trimmed to insert at both ends. Bases 137 to 174 (complement) show homology to 25e4.1 bases 245 to 282 (38 in longest run, maxScore = 114). (CA)7 308 to 321, (CA)15 356 to 385, (CA)9 388 to 405, (CA)6 412 to 423. Forward primer has 2 mismatches to 25e4.1, reverse primer apparently outside region of homology. Bases 143 to 177 show homology to 15b3.1 bases 276 to 310 (complement) (35 in longest run, maxScore = 113).
Type	PCR SSR
PCR primers	Forward AggcccAcAcAATcTcTTcg at 242 length 20 bases, Tm 64 Reverse TggggATgAATTgTgTgcTc at 431 length 20 bases, Tm 64
SSR size	209 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear.
Sequence	WMC520
Source Species	Triticum aestivum