Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC519

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC519
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 22b3 Donor: Bright, Zeneca Trimmed to insert at both ends. Bases 6 to 46 (complement) show homology to 4f4 bases 119 to 159 (41 in longest run, maxScore = 54). Take care, reverse primer has 2 mismatches to 4f4, forward primer is homologous. (GT)4 59 to 66, (GT)7 69 to 82, (GT)11 105 to 126.
Type	PCR SSR
PCR primers	Forward cggcggcAgggAcTTT at 20 length 16 bases, Tm 66 Reverse cccgcgTcATTTccTcAA at 147 length 18 bases, Tm 65
SSR size	145 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear.
Sequence	WMC519
Source Species	Triticum aestivum