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GrainGenes Probe Report: WMC516

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC516  [ Marker Report ]
Locus	Xwmc516-4A wmc516
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 22d1 Donor: Bright, Zeneca Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 111 bases located on 3A, 125 bases located on 3D, 129 bases located on 4A, 157 bases located on 5D, 217 bases located on 2A, 247 bases located on 3A, 282 bases located on 2A, 289 bases located on 5D, 319 bases located on 3D, 438 bases located on 3D, 555 bases located on 3D, 830 bases located on 3A Trimmed to insert at both ends. Bases 108 to 123 (complement) show homology to 10a10 bases 127 to 142 (16 in longest run, maxScore = 25). Reverse primer has mismatch to 10a10, forward primer has no apparent homology to 10a10. (CA)12 21 to 44, (CA)6 94 to 105.
Type	PCR SSR
PCR primers	Forward gggccAcgAATAAAcAg at 3 length 17 bases, Tm 57 Reverse gAcTcgcAAcTAggggT at 113 length 17 bases, Tm 55
SSR size	127 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product (130 and 150 bases).
Sequence	WMC516
Source Species	Triticum aestivum