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GrainGenes Probe Report: WMC515

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC515
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 22c1 Donor: Bright, Zeneca Trimmed to insert at both ends. Bases 160 to 182 show homology to 5f11 bases 131 to 153 (23 in longest run, maxScore = 73). (CA)16 90 to 121.
Type	PCR SSR
PCR primers	Forward TTgTgcgAAgAccgATAATAAc at 6 length 22 bases, Tm 61 Reverse TggAAATgTcATgcgTgTAgTA at 175 length 22 bases, Tm 61
SSR size	191 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear;
Sequence	WMC515
Source Species	Triticum aestivum