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GrainGenes Probe Report: WMC513

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC513  [ Marker Report ]
Locus	Xwmc513 Xwmc513-4A wmc513
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24h9 Donor: Byrne, Colorado State Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 226 bases located on 4A Trimmed to Ssp1 at 5' end and to insert at 3' end (3' linker corrupted ) . (CA)21 118 to 159.
Type	PCR SSR
PCR primers	Forward TgAATTgAATcTggTTgcgg at 58 length 20 bases, Tm 64 Reverse TggcAATTcAcAggcAcATA at 247 length 20 bases, Tm 63
SSR size	209 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC513
Source Species	Triticum aestivum