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GrainGenes Probe Report: WMC512

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC512
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24a9 Donor: Byrne, Colorado State Sequence received in 99. Trimmed to insert at 3' end, 5' end not located. Bases 142 to 168 show homology to 6A1 bases 611 to 637 (27 in longest run, maxScore = 68). Reverse primer has 2 mismatches to 6A1, forward primer does not match. (TC)7 187 to 200.
Type	PCR SSR
PCR primers	Forward AggggTTcTTTcAAgTTcgc at 12 length 20 bases, Tm 62 Reverse cccAgggAgATggAgAgTTT at 201 length 20 bases, Tm 62
SSR size	209 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC512
Source Species	Triticum aestivum