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GrainGenes Probe Report: WMC504

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC504
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 20c8 Donor: Byrne, Colorado State Trimmed to insert at 5' end, 3' end not located. Bases 32 to 63 (complement) show homology to 25d6 bases 67 to 98 (32 in longest run, maxScore = 56), reverse primer has 2 mismatches to 25d6, forward primer has no matches. (CT)7 67 to 80, (CT)7 85 to 98.
Type	PCR SSR
PCR primers	Forward gTTcTgTcgcAcgcATTcAA at 15 length 20 bases, Tm 65 Reverse cATgccTgcAggTcgAcTcT at 141 length 20 bases, Tm 66
SSR size	146 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product. t 51C anneal, product(100-120130-226 bases).
Sequence	WMC504
Source Species	Triticum aestivum