Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC503

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC503  [ Marker Report ]
Locus	Xwmc503-2D Xwmc503 wmc503 wmc503-2D wmc503-3A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 20b8 Donor: Byrne, Colorado State Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 237 bases located on 2D, 5B Trimmed to insert at both ends. (GT)11 112 to 133, (GT)18 192 to 227.
Type	PCR SSR
PCR primers	Forward gcAATAgTTcccgcAAgAAAAg at 55 length 22 bases, Tm 64 Reverse ATcAAcTAccTccAgATcccgT at 270 length 22 bases, Tm 63
SSR size	237 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC503
Source Species	Triticum aestivum