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GrainGenes Probe Report: WMC502

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC502
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 20e7 Donor: Byrne, Colorado State Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 116 to 153 (complement) show homology to 25c7 bases 218 to 255 (38 in longest run, maxScore = 93). Bases 263 to 357 show homology to 24a8 bases 23 to 117 (complement) (95 in longest run, maxScore = 48). (GT)14 262 to 289, (CT)14 232 to 259.
Type	PCR SSR
PCR primers	Forward cgTTgTgggTgTTATAcccTAc at 83 length 22 bases, Tm 60 Reverse AAgAAgggAgAATcTAgAAgcc at 327 length 22 bases, Tm 59
SSR size	266 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product.
Sequence	WMC502
Source Species	Triticum aestivum