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GrainGenes Probe Report: WMC501

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC501  [ Marker Report ]
Locus	Xwmc501.2 Xwmc501.1
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 20b7 Donor: Byrne, Colorado State Trimmed to insert at 3' end, 5' end not located. Bases 241 to 295 show homology to 4f3 bases 112 to 166 (55 in longest run, maxScore = 61), forward primer is not within this region of homology, reverse primer matches 4f3. (GT)30 244 to 303.
Type	PCR SSR
PCR primers	Forward cTcggcTTcAcTATcgTcgTcT at 105 length 22 bases, Tm 65 Reverse TAcTTgTcAcATcggcccTcTT at 306 length 22 bases, Tm 65
SSR size	223 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC501
Source Species	Triticum aestivum