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GrainGenes Probe Report: WMC499

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC499  [ Marker Report ]
Locus	Xwmc499
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25g11 Donor: Serasem Trimmed to insert at both ends (NB 5' linker corrupted). Bases 253 to 270 (complement) show homology to 2f12 bases 141 to 158 (18 in longest run, maxScore = 19). (GT)29 176 to 233.
Type	PCR SSR
PCR primers	Forward ggAAcTcTgTggTccAccTgA at 98 length 21 bases, Tm 64 Reverse cggTgTgAccTAgcAcgAAcT at 272 length 21 bases, Tm 64
SSR size	195 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide ((150-190 bases)
Sequence	WMC499
Source Species	Triticum aestivum