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GrainGenes Probe Report: WMC498

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC498  [ Marker Report ]
Locus	Xwmc498-2B wmc498 wmc498-2B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25e11 Donor: Serasem Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 136 bases located on 2B Trimmed to insert at both ends. Bases 1 to 32 show homology to 2b1 bases 76 to 107 (32 in longest run, maxScore = 36). (GT)14 50 to 77, (GT)6 80 to 91, (GT)6 94 to 105, (GC)5 106 to 115.
Type	PCR SSR
PCR primers	Forward cgATgAAgAgAgccATcAAAA at 27 length 21 bases, Tm 62 Reverse TgAcATTccggTAggTcAgTT at 142 length 21 bases, Tm 61
SSR size	136 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide (134 bases - gives null alleles).
Sequence	WMC498
Source Species	Triticum aestivum