Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC497

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC497  [ Marker Report ]
Locus	Xwmc497.2 [ Show all 7 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25a11 Donor: Serasem Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 222 bases located on 4A, 250 bases located on 7D Trimmed to insert at both ends. (GT)22 262 to 305.
Type	PCR SSR
PCR primers	Forward cccgTggTTTTcTTTccTTcT at 109 length 21 bases, Tm 63 Reverse AAcgAcAgggATgAAAAgcAA at 307 length 21 bases, Tm 64
SSR size	219 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (210-220 bases).
Sequence	WMC497
Source Species	Triticum aestivum