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GrainGenes Probe Report: WMC495

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC495
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25b10 Donor: Serasem Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 165 to 199 (complement) show homology to 25e4.1 bases 194 to 228 (35 in longest run, maxScore = 111). Forward primer is outside this region of homology, reverse primer has a mismatch to to 25e4.1. (GA)13 72 to 97, (GT)9 54 to 71.
Type	PCR SSR
PCR primers	Forward AcgTTgTgTATAcATgcgAgAA at 14 length 22 bases, Tm 60 Reverse AgcgAgAAcAAAccTAAggAgA at 250 length 22 bases, Tm 61
SSR size	258 bases expected, Chinese Spring
Amplification Conditions	51C anneal, smear.
Sequence	WMC495
Source Species	Triticum aestivum