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GrainGenes Probe Report: WMC488

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC488  [ Marker Report ]
Locus	Xwmc488b-7A [ Show all 7 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25e8 Donor: Serasem Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 106 bases located on 7A Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)14 70 to 97, (GT)9 100 to 117.
Type	PCR SSR
PCR primers	Forward AAAgcAcAAccAgTTATgccAc at 39 length 22 bases, Tm 63 Reverse gAAccATAgTcAcATATcAcgAgg at 151 length 24 bases, Tm 62
SSR size	136 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (120-140).
Sequence	WMC488
Source Species	Triticum aestivum