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GrainGenes Probe Report: WMC477

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC477  [ Marker Report ]
Locus	Xwmc477-2B Xwmc0477-2B wmc477-2B wmc477 Xwmc477
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25b4 Donor: Jizeng, Beijing Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 170 bases located on 2B Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)16 118 to 149.
Type	PCR SSR
PCR primers	Forward cgTcgAAAAccgTAcAcTcTcc at 10 length 22 bases, Tm 65 Reverse gcgAAAcAgAATAgcccTgATg at 155 length 22 bases, Tm 65
SSR size	167 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC477
Source Species	Triticum aestivum