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GrainGenes Probe Report: WMC466

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC466
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 21f10 Donor: Dupont Trimmed to insert at 5' end and to Ssp1 at 3' end (NB 5' linker corrupted). Bases 24 to 79 (complement) show homology to 24c3 bases 173 to 228 (56 in longest run, maxScore = 20). (CA)22 36 to 79, (CA)19 81 to 118.
Type	PCR SSR
PCR primers	Forward gAATgcTgTAgAggAcAgAAc at 16 length 21 bases, Tm 56 Reverse gAAAcAAAATAgcccAgATg at 121 length 20 bases, Tm 57
SSR size	124 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide (96-102 plus smaller bands).
Sequence	WMC466
Source Species	Triticum aestivum