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GrainGenes Probe Report: WMC465

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC465
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 21c10 Donor: Dupont Trimmed to insert at both ends. Bases 68 to 108 show homology to 1C4 bases 239 to 279 (41 in longest run, maxScore = 80). (GT)13 155 to 180.
Type	PCR SSR
PCR primers	Forward gccAccgATgTAAgTggATgT at 103 length 21 bases, Tm 64 Reverse AAggTgTgTgcgATgggATT at 196 length 20 bases, Tm 64
SSR size	113 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product ( very stuttery 100-120 bases).
Sequence	WMC465
Source Species	Triticum aestivum