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GrainGenes Probe Report: WMC462

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC462
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 21h7 Donor: Dupont Trimmed to insert at 5' end and to Ssp1 at 3' end. (CA)19 260 to 297.
Type	PCR SSR
PCR primers	Forward gTgTTTTAgcAgTTAcTccgT at 108 length 21 bases, Tm 56 Reverse AATAATggTTTcTAAggTggg at 298 length 21 bases, Tm 56
SSR size	211 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product (circa 211 bases).
Sequence	WMC462
Source Species	Triticum aestivum