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GrainGenes Probe Report: WMC461

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC461
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 21g7 Donor: Dupont Trimmed to insert at both ends (NB 5' linker corrupted.) . Bases 84 to 103 (complement) show homology to 5b8 bases 299 to 318 (20 in longest run, maxScore = 73). (CA)5 20 to 29, (CA)9 102 to 119, (CA)7 197 to 210.
Type	PCR SSR
PCR primers	Forward cccgAcAccAAAggAgTAgg at 33 length 20 bases, Tm 64 Reverse cAcAgAAgcAcAAcAAgcgA at 212 length 20 bases, Tm 63
SSR size	199 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product (180-200).
Sequence	WMC461
Source Species	Triticum aestivum