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GrainGenes Probe Report: WMC460

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC460
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 21e7 Donor: Dupont Trimmed to insert at both ends. Bases 59 to 100 show homology to 22b10 bases 170 to 211 (42 in longest run, maxScore = 53). (GA)4 2 to 9, (GA)6 28 to 39, (GA)7 42 to 55, (GA)26 143 to 194.
Type	PCR SSR
PCR primers	Forward gAgAAAgggAgAgggAccAAc at 78 length 21 bases, Tm 63 Reverse gccTcccTcTccccTTgTAAc at 198 length 21 bases, Tm 65
SSR size	141 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product (400 bases).
Sequence	WMC460
Source Species	Triticum aestivum