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GrainGenes Probe Report: WMC457

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC457  [ Marker Report ]
Locus	Xwmc457-4D Xwmc457 wmc457
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2d5.1 Donor: IGS BioTech Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 164 bases located on 4D, 4A No overlap between forward and reverse sequences. Multple inserts in this clone, this is the first insert. Trimmed to insert at both ends. (CA)13 342 to 367.
Type	PCR SSR
PCR primers	Forward cTTccATgAATcAAAgcAgcAc at 243 length 22 bases, Tm 64 Reverse cATccATggcAgAAAcAATAgc at 380 length 22 bases, Tm 63
SSR size	159 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC457
Source Species	Triticum aestivum