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GrainGenes Probe Report: WMC454

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC454
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2g2 Donor: IGS BioTech Trimmed to Ras1 at 5' end and to insert at 3' end. Bases 96 to 105 show homology to 6b11 bases 325 to 334 (10 in longest run, maxScore = 20). (TG)6 61 to 72, (GT)5 80 to 89, (GT)8 126 to 141, (GT)6 162 to 173, (GT)21 246 to 287.
Type	PCR SSR
PCR primers	Forward ATTATTTAgAAcAgATTgcggT at 35 length 22 bases, Tm 56 Reverse AAcATggTgcATATTTAgTgAT at 328 length 22 bases, Tm 56
SSR size	315 bases expected, Chinese Spring
Sequence	WMC454
Source Species	Triticum aestivum