Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC448

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC448
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2b1 Donor: IGS BioTech Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 153 to 165 (complement) show homology to 19b4 bases 216 to 228 (13 in longest run, maxScore = 25). Bases 76 to 107 show homology to 25e11 bases 1 to 32 (32 in longest run, maxScore = 36). (GT)12 125 to 148.
Type	PCR SSR
PCR primers	Forward ATcATAcATAgATggTcgggcA at 6 length 22 bases, Tm 63 Reverse ggccAgTcgcTcTATATcTTTg at 155 length 22 bases, Tm 62
SSR size	171 bases expected, Chinese Spring
Amplification Conditions	61C anneal, complex product.
Sequence	WMC448
Source Species	Triticum aestivum