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GrainGenes Probe Report: WMC441

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
WMC441  [ Marker Report ]
Locus
Xwmc441-2B
[ Show all 8 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 24d6
Donor: ITCF
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 159 bases located on 2B
Trimmed to insert at both ends. Bases 144 to 164 show homology to 17h7 bases 131 to 151 (21 in longest run, maxScore = 38). (CA)10 53 to 72, (CG)9 73 to 90, (CT)9 35 to 52.
Type
PCR
SSR
PCR primers
Forward TccAgTAgAgcAccTTTcATT at 5 length 21 bases, Tm 58
Reverse ATcAcgAAgATAAAcAAAcgg at 142 length 21 bases, Tm 58
SSR size
158 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic on acrylamide (complex).
Sequence
WMC441
Source Species
Triticum aestivum