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GrainGenes Probe Report: WMC440

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
WMC440
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 24c6
Donor: ITCF
Trimmed to insert at both ends (NB 3' end corrupted). (GT)12 74 to 97.
Type
PCR
SSR
PCR primers
Forward ggAAAccAAAAAgTcccgA at 13 length 19 bases, Tm 62
Reverse ggTgggAggggAcTAgcAT at 98 length 19 bases, Tm 63
SSR size
104 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product.
Sequence
WMC440
Source Species
Triticum aestivum