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GrainGenes Probe Report: WMC434

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC434  [ Marker Report ]
Locus	Xwmc434-4D [ Show all 9 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24c4 Donor: ITCF Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 165 bases located on 5D, 175 bases located on 2B Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 79 to 107 show homology to 2e8 bases 345 to 373 (29 in longest run, maxScore = 53). (GT)12 330 to 353.
Type	PCR SSR
PCR primers	Forward ggAgccTgATTAggcTggAc at 195 length 20 bases, Tm 63 Reverse AgccAAAcAgccAAcAgAgT at 356 length 20 bases, Tm 62
SSR size	181 bases expected, Chinese Spring
Amplification Conditions	61C anneal, stuttery smear.
Sequence	WMC434
Source Species	Triticum aestivum