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GrainGenes Probe Report: WMC433

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC433
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24a4 Donor: ITCF Trimmed to insert at both ends. Bases 118 to 159 show homology to 14c4 bases 348 to 389 (42 in longest run, maxScore = 63). Bases 1 to 196 show homology to 24d4 bases 1 to 196 (196 in longest run, maxScore = 127), duplicate with 24d4, 24a4 has priority. (GT)7 24 to 37, (GT)7 56 to 69, (GT)11 82 to 103.
Type	PCR SSR
PCR primers	Forward gATgAATTgTATgcTcgggg at 2 length 20 bases, Tm 62 Reverse ccTTAggTTTgTTTccgcTc at 112 length 20 bases, Tm 60
SSR size	130 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; tested 51C anneal, no product.
Sequence	WMC433
Source Species	Triticum aestivum