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GrainGenes Probe Report: WMC429

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC429  [ Marker Report ]
Locus	Xwmc429-1D Xwmc429 wmc429
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24c3 Donor: ITCF Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 229 bases located on 1D Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 274 to 296 show homology to 11b12 bases 459 to 481 (23 in longest run, maxScore = 69). (GT)31 156 to 217, Bases 173 to 228 show homology to 21f10 bases 24 to 79 (complement) (56 in longest run, maxScore = 20). Bases 6 to 34 show homology to 25c9 bases 322 to 350 (complement) (29 in longest run, maxScore = 25).
Type	PCR SSR
PCR primers	Forward cgTAAAgATTTTcATTTggcg at 41 length 21 bases, Tm 61 Reverse AAcggcAgcTTgAAAAcATAg at 245 length 21 bases, Tm 61
SSR size	225 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC429
Source Species	Triticum aestivum