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GrainGenes Probe Report: WMC426

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC426  [ Marker Report ]
Locus	Xwmc426-7B wmc426 Xwmc426 wmc426-7B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 21f6 Donor: Langridge, CRC Australia Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 208 bases located on 2A, 2B No trimming done to sequence at Agrogene, ends not located. (CA)4 111 to 118, (CA)15 121 to 150. Bases 6 to 47 show homology to 21h9 bases 132 to 173 (complement) (42 in longest run, maxScore = 64). Duplicate with 21h9, 21f6 has priority.
Type	PCR SSR
PCR primers	Forward gAcgATcgTTTcTccTAcTTTA at 8 length 22 bases, Tm 57 Reverse AcTAcAcAAATgAcTgcTgcTA at 190 length 22 bases, Tm 57
SSR size	204 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide (200-206 bases above a smaller smear).
Sequence	WMC426
Source Species	Triticum aestivum