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GrainGenes Probe Report: WMC425

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC425
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 21h4 Donor: Langridge, CRC Australia No trimming done to sequence at Agrogene, ends not located. Bases 200 to 247 show homology to 14c4 bases 369 to 416 (48 in longest run, maxScore = 89). (GT)7 89 to 102, (CA)8 370 to 385, (GT)16 109 to 140, (GT)10 145 to 164. Bases 153 to 180 show homology to 25e4.1 bases 101 to 128 (28 in longest run, maxScore = 110).
Type	PCR SSR
PCR primers	Forward AATAccgcAcAgATgcgTAAgg at 38 length 22 bases, Tm 65 Reverse cgcATgcAAcATATgcATcAAT at 271 length 22 bases, Tm 66
SSR size	254 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product.
Sequence	WMC425
Source Species	Triticum aestivum