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GrainGenes Probe Report: WMC424

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC424
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 21g4 Donor: Langridge, CRC Australia No trimming done to sequence at Agrogene, ends not located. Bases 15 to 52 show homology to 13c5 bases 1 to 38 (38 in longest run, maxScore = 52). (GT)26 88 to 139, (GT)8 156 to 171.
Type	PCR SSR
PCR primers	Forward gATTAcTATcAAgcTcgAggTgc at 40 length 23 bases, Tm 61 Reverse TTcAggTAAccAcTAgAgTATgTcc at 177 length 25 bases, Tm 60
SSR size	162 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide (complex, polymorphism at 330 bases possibly others).
Sequence	WMC424
Source Species	Triticum aestivum