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GrainGenes Probe Report: WMC423

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC423
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 21a4 Donor: Langridge, CRC Australia No trimming done to sequence at Agrogene, ends not located. Bases 40 to 67 show homology to 11f10 bases 481 to 508 (28 in longest run, maxScore = 53). (GT)25 103 to 152.
Type	PCR SSR
PCR primers	Forward cAcgcgAgTTTTAcgAgTTcA at 17 length 21 bases, Tm 64 Reverse TggAcTAcgcAcccccTgT at 154 length 19 bases, Tm 66
SSR size	154 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product (115 bases).
Sequence	WMC423
Source Species	Triticum aestivum