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GrainGenes Probe Report: WMC419

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC419  [ Marker Report ]
Locus	Xwmc419-1B [ Show all 8 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 21e1 Donor: Langridge, CRC Australia Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 145 bases located on 1B No trimming done to sequence at Agrogene, ends not located. Bases 121 to 143 (complement) show homology to 1H5 bases 223 to 245 (23 in longest run, maxScore = 25). (GA)16 111 to 142.
Type	PCR SSR
PCR primers	Forward gTTTcggATAAAAccggAgTgc at 36 length 22 bases, Tm 65 Reverse AcTAcTTgTgggTTATcAccAgcc at 153 length 24 bases, Tm 65
SSR size	141 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide (122-168 bases)
Sequence	WMC419
Source Species	Triticum aestivum