GrainGenes Probe Report: WMC416
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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.
Probe
WMC416 [ Marker Report ]
Locus
Xwmc416-6D Xwmc416 Xwmc416-1B wmc416 wmc416-1B
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 23a3 Donor: Romestant, RAGT Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 223 bases located on 6D Trimmed to Ssp1 at both ends of insert. (CA)11 239 to 260, (CA)11 263 to 284, (CA)8 287 to 302.
Type
PCR SSR
PCR primers
Forward AgcccTTTcTAccgTgTTTcTT at 169 length 22 bases, Tm 62
Reverse TATggTcgATggAcTgTcccTA at 367 length 22 bases, Tm 63
SSR size
220 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide.
Sequence
WMC416
Source Species
Triticum aestivum
GrainGenes Probe Report: WMC416
[Submit comment/correction][What is a probe?]
Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.
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