Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC410

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC410  [ Marker Report ]
Locus	Xwmc410 wmc410 wmc410-5A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10g4 Donor: Gale, John Innes Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)26 69 to 120. Bases 62 to 137 show homology to 2d1 bases 1 to 76 (complement) (76 in longest run, maxScore = 58). True duplicate with 2d1, 10g4 has priority.
Type	PCR SSR
PCR primers	Forward ggAcTTgAAAggAAgcTTgTgA at 21 length 22 bases, Tm 63 Reverse cATggATggcATgcAgTgT at 118 length 19 bases, Tm 64
SSR size	116 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC410
Source Species	Triticum aestivum