Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC407

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC407  [ Marker Report ]
Locus	Xwmc407 [ Show all 11 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10a2 Donor: Gale, John Innes Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 137 bases located on 2A Trimmed to insert at 5' end and to Rsa1 at 3' end. (GA)16 100 to 131.
Type	PCR SSR
PCR primers	Forward ggTAATTcTAggcTgAcATATgcTc at 30 length 25 bases, Tm 61 Reverse cATATTTccAAATccccAAcTc at 143 length 22 bases, Tm 61
SSR size	135 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (136-138 bases).
Sequence	WMC407
Source Species	Triticum aestivum