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GrainGenes Probe Report: WMC404

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC404
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 22c10 Donor: Romestant, RAGT Trimmed to insert at both ends. (CT)4 34 to 41, (CA)25 56 to 105.
Type	PCR SSR
PCR primers	Forward AcgAgTTggTTgTgTcgccT at 8 length 20 bases, Tm 65 Reverse AgcATATgcATTTcgTTTggg at 160 length 21 bases, Tm 64
SSR size	173 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC404
Source Species	Triticum aestivum