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GrainGenes Probe Report: WMC403

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC403
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 22h9 Donor: Romestant, RAGT Trimmed to insert at 5' end, 3' end not located. Bases 71 to 131 show homology to 11h2 bases 225 to 285 (61 in longest run, maxScore = 92) - to one side of microsatellite. (GT)12 139 to 162. Reverse primer has no apparent homology with 11h2
Type	PCR SSR
PCR primers	Forward ggcAAAgAAgcAcAAgcgAg at 70 length 20 bases, Tm 65 Reverse gcgTAAgcAAgAgcAcAgcA at 165 length 20 bases, Tm 65
SSR size	115 bases expected, Chinese Spring
Amplification Conditions	tested 51C anneal, no product.
Sequence	WMC403
Source Species	Triticum aestivum