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GrainGenes Probe Report: WMC402

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
WMC402  [ Marker Report ]
Locus
Xwmc402
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 22g9
Donor: Romestant, RAGT
Trimmed to insert at both ends. Bases 109 to 142 show homology to 11f8 bases 401 to 434 (34 in longest run, maxScore = 52). (CA)15 66 to 95. Bases 120 to 138 show homology to 2h2 bases 155 to 173 (complement) (19 in longest run, maxScore = 38).
Type
PCR
SSR
PCR primers
Forward gcccAAAATgTcAcggATgT at 16 length 20 bases, Tm 65
Reverse TAgcTTggTTTggAgcgAgg at 135 length 20 bases, Tm 64
SSR size
139 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product.
Sequence
WMC402
Source Species
Triticum aestivum