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GrainGenes Probe Report: WMC390

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC390  [ Marker Report ]
Locus	Xwmc390.1 Xwmc390.2
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10b10 Donor: Jacquemin, Gembloux Trimmed to insert at 5' end , 3' end not located. Bases 1 to 40 (complement) show homology to 8a12 bases 123 to 162 (40 in longest run, maxScore = 48). (GT)16 84 to 115.
Type	PCR SSR
PCR primers	Forward TgcAAATgccATggTTgTAgTA at 7 length 22 bases, Tm 63 Reverse cAgAcgggTTgcTgcTAATAAA at 169 length 22 bases, Tm 63
SSR size	184 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC390
Source Species	Triticum aestivum